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5cm x 100um C18 UHPLC trap column with 2 x nanoZero® fittings compatible with nanoViper™ 3 Pack

Chromatographic trap columns are essential in proteomic UHPLC–MS workflows, improving analyte focusing and enabling rapid sample loading with in-line sample clean-up to protect analytical columns from blockages and contamination while reducing ion suppression. These benefits extend column lifetime and reduce maintenance, supporting robust, high-throughput, and reproducible proteomic analyses. Despite these advantages, trap columns traditionally sacrifice peptide and protein identifications compared to direct injection. The NanoShield™ C18 5 cm × 100 μm trap column eliminates this trade-off – delivering direct injection-level performance; providing superior peptide identifications, peak quality, and hydrophilic peptide coverage compared to other traps.

• Next to no peptide ID loss

• Extends column life, reduces maintenance costs

• Improved reproducibility across thousands of injections

• Enables hundreds of samples per day

Ideal for

• Fast sample loading

• Reversed-flush workflows

• Bottom-up discovery workflows

Aurora Gen 3 columns have been discontinued from the manufacturer, but we still have a selection of the most popular sizes and configurations, get them while they last! Great value on them, as prices have increased on the newest Gen 4 columns.

If the form does not work for your selected column, it is out of stock. Please refer to the newest list of Gen 4 column on the other page.

IonOpticks’ new generation of UHPLC columns reimagines what proteomics separation technology can achieve.

Find here the full list of the latest Gen 4 Aurora columns from Ion Opticks. We strive to keep these very popular items in stock, but importing is a tricky business lately, so plan in advance important purchases with us to ensure availability.

We are located just north of Boston, trying to keep the single cell proteomics community supplied with columns and other hardware.

These columns are fritted glass capillaries with several options for inlet and outlet fittings to match your system exactly for ease of installation and continuity across platforms. All of these require some type of nanospray tip connection, made easy with the male 1/16” outlet fitting to many tip adaptors and reducing union fittings.

The columns are expertly pulled and etched to a precision tip of <4-degree angle for super high sensitivity analysis. Available with inlet fittings or as a bare glass column with various ID and lengths. Packed with 1.6um C18 media. PEEK sleeves protect the tip. Available in single, 5 packs and 10 packs to maximize your budget.

These columns are preassembled in ColuMate cartridges. Available in a classic pulled tip style, or a blunt end with options for different nanospray tips with a post column liquid junction.

The ColuMate™ System is an easy-to-use capillary column heating solution for nano-LC/HPLC system. It enables simultaneous but independent control of two heating channels, with both manual and remote control for maximum convenience. Each channel provides 30–60 °C of consistent column heating (±0.5 °C) from inlet to outlet. The reusable heating chamber features a simple clamshell design for easy installation and exchange of ColuMate-Ready columns. Our cartridge-based approach is suitable for most column sizes and can be adapted to many third-party columns.

Equalizer 1 is digested and ready to run.

Equalizer 1 is derived from a NIST Standard Reference Material. (NIST SRM 1950)

Simply add your mobile phase A to Equalizer 1 and run. For example the 20 microgram lot 200 microliters will make a 100 nanogram per microliter solution.