For covalent ligand immobilization via their primary amine residues
MagReSyn? Carboxyl is a magnetic polymeric microparticles support designed for covalent immobilization of primary amine-containing ligands through carbodiimide-succinimidyl ester activation and subsequent amide bond formation. Various strategies for the activation of carboxylate particles have been documented, but the most commonly used method for coupling proteins and other primary amine-containing ligands is through activation by EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) in an aqueous reaction. This reaction can be either single-step or a two-step reaction requiring the addition of N-hydroxysuccinimide (NHS) or the more stable sulfo-NHS yielding NHS ester or sulfo NHS ester intermediates.
Please note that coupling of proteins through NHS-EDC activation can be tricky, requires fresh reagents, and is prone to interference from a range of routine buffers used in life-sciences. For simpler coupling of your ligand we would recommend using MagReSyn? Amine with glutaraldehyde or epoxide activation.?